Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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First report of Pythium root rot caused by Pythium mastophorum on parsley in the United States
Author: Tsuchida C, Mauzey S, Hatlen R, Miles TD, Koike ST.We believe this is the first report of P. mastophorum infecting parsley in California. P. mastophorum has been reported on parsley only in southeastern Australia (Petkowski et al., 2013). In California, this species has been reported on celery (Vazquez et […]
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Rapid and sensitive detection of mink circovirus by recombinase polymerase amplification
Author: Ge J, Shi Y, Cui X, Gu S, Zhao L, Chen H.In this study, we developed a nested PCR and established a novel recombinase polymerase amplification (RPA) assay for MiCV detection. Sensitivity analysis showed that the detection limit of nested PCR and RPA assay was 101 copies/reaction, and these methods were […]
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Author: Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA.By combining Cas12a ssDNase activation with isothermal amplification, we create a method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR), which achieves attomolar sensitivity for DNA detection.
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Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis.
Author: Shahin K, Ramirez-Paredes JG, Harold G, Lopez-Jimena B, Adams A, Weidmann M.The RPA gave quicker results in approximately 6 min in contrast to the qPCR that needed about 90 min to reach the same detection limit, taking only 2.7-3 min to determine Fno in clinical samples. Moreover, RPA was more tolerant […]
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Real-time RPA assay for rapid detection and differentiation of wild-type pseudorabies and gE-deleted vaccine viruses
Author: Wang J, Liu L, Wang J, Pang X, Yuan W.The analytical sensitivity of the assay was 102 copies for gB and gE genes. The dual real-time RPA demonstrated a 100% diagnostic agreement with the real-time PCR on 4 PRV strains and 37 clinical samples.
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Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays
Author: Hou P, Wang H, Zhao G, He C, He H.The assay performance on acute-phase high fever clinical samples collected from cattle with no vaccine against IBRV, which were suspected to be infected with IBRV, was validated by detecting 24 fecal, 36 blood, 38 nasal swab and 8 tissue specimens, […]
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Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene
Author: Martorell S, Palanca S, Maquieira A, Tortajada-Genaro LA.Herein, the detection of mutations in PIK3CA gene, such as p.E545K, and p.H1047L, is presented. The main element was an oligonucleotide (dideoxycytidine functionalized at 3′-end) which matched with wild-type sequence in the target locus.
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Rapid and visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick.
Author: Guimin Z, Hongmei W, Peili H, Chengqiang H, Hongbin H.The assay was specific, as it did not amplify genomes from five other Mycobacterium and five pathogenic enteric bacteria. Then, 612 clinical samples (320 fecal and 292 serum) were assessed by RPA-LFD, qPCR and ELISA assays respectively, also the established […]
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Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus
Author: Hou P, Zhao G, Wang H, He C, Huan Y, He H.The result showed that the coincidence rate of BEFV LFD-RPA and real-time qPCR was 96.09% (123/128), which was higher than conventional RT-PCR. The RPA combined with LFD assay probably provides a rapid and sensitive alternative for diagnosis of BEFV infections […]
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Real-time reverse transcription recombinase polymerase amplification assay for rapid detection of porcine epidemic diarrhea virus
Author: Wang J, Zhang R, Wang J, Han Q, Liu L, Li Y, Yuan W.The assay performance was evaluated by testing 76 clinical samples. PEDV RNA positive rate was 55.3% (42/76) by RT-RPA and 59.2% (45/76) by real-time RT-PCR. The diagnostic agreement between the two assays was 96.1% (73/76), and the R2 value of […]