Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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RPA using a multiplexed cartridge for low cost point of care diagnostics in the field
Author: Ereku LT, Mackay RE, Craw P, Naveenathayalan A, Stead T, Branavan M, Balachandran W.This paper shows the results from two separate experiments: the first using the RPA control nucleic acid, the second showing successful amplification from Chlamydia Trachomatis.
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Author: Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA.By combining Cas12a ssDNase activation with isothermal amplification, we create a method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR), which achieves attomolar sensitivity for DNA detection.
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Recombinase polymerase amplification combined with lateral flow dipstick for equipment-free detection of Salmonella in shellfish.
Author: Gao W, Huang H, Zhu P, Yan X, Fan J, Jiang J, Xu J.The RPA-LFD was able to function at 30-45 °C, and at the temperature of 40 °C, it only took 8 min of amplification to reach the test threshold of amplicons. The established method had both a good specificity and a […]
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Development of a recombinase polymerase amplification assay for Vibrio parahaemolyticus detection with an internal amplification control.
Author: Yang H, Wei S, Gooneratne R, Mutukumirad AN, Ma X, Tang S, Wu X.The sensitivity of the assay was determined as 3×103 CFU/mL, which is decidedly more sensitive than the established PCR method. This method was then used to test seafood samples which were collected from local market. 7 out of 53 different […]
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Molecular Detection of the Theileria Annulata in Cattle from different regions of Punjab, Pakistan using Recombinase Polymerase Amplification and PCR
Author: Hassan MA, Liu J, Sajid MS, Mahmood A, Zhao SY, Abbas Q, Guan G, Yin H, Luo J.All 274 samples were screened using conventional PCR and 21 (7.66%) samples were positive for T. annulata. All the samples that were RPA positive but PCR negative were sequenced, which confirmed the results of RPA. The highest positive rate was […]
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Duplex recombinase polymerase amplification assays incorporating competitive internal controls for bacterial meningitis detection.
Author: Higgins O, Clancy E, Forrest MS, Piepenburg O, Cormican M, Boo TW, McGuinness C, O'Sullivan N, Cafferty D, Cunney R, Smith TJ.Clinical performance of each internally controlled duplex RPA assay was evaluated by testing 64 archived PCR-positive clinical samples. Compared to real-time PCR, all duplex RPA assays demonstrated 100% diagnostic specificity, with diagnostic sensitivities of 100%, 86.3% and 100% for the […]
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Rapid detection of potyviruses from crude plant extracts
Author: Silva G, Oyekanmi J, Nkere CK, Bomer M, Kumar L, Seal SE.The direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be […]
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Heterogeneous asymmetric recombinase polymerase amplification (haRPA) for rapid hygiene control of large-volume water samples
Author: Elsasser D, Ho J, Niessner R, Tiehm A, Seidel M.Field measurements were conducted to test the developed system for hygiene online monitoring under realistic conditions. We could show that this system allows the detection of artificial contaminations of bacteriophage PhiX174 in drinking water pipelines.
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Development of a lateral flow recombinase polymerase assay for the diagnosis of Schistosoma mansoni infections
Author: Poulton K, Webster B.The 28S LF-RPA assay’s lower limit of detection was 10pg DNA with the lower test parameters permitting sufficient amplification being 6 min and 25°C. Optimal assay parameters were 40-45°C and 10 min with an analytical sensitivity of 102 copies of DNA.
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Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis.
Author: Shahin K, Ramirez-Paredes JG, Harold G, Lopez-Jimena B, Adams A, Weidmann M.The RPA gave quicker results in approximately 6 min in contrast to the qPCR that needed about 90 min to reach the same detection limit, taking only 2.7-3 min to determine Fno in clinical samples. Moreover, RPA was more tolerant […]