Publications
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A recombinase polymerase amplification-based assay for rapid detection of African swine fever virus.
Author: Wang J, Wang J, Geng Y, Yuan W.Tests of recombinant plasmid-spiked serum samples revealed that RPA and real-time PCR had the same diagnostic rate. The RPA assay, which is simple, cost-effective, and fast, is a promising alternative to real-time PCR for ASFV detection.
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Real-time isothermal detection of Abalone herpes-like virus and red-spotted grouper nervous necrosis virus using recombinase polymerase amplification
Author: Gao F, Jiang J-Z, Wang J-Y, Wei H-Y• This is the first study to use RPA to detect AbHV and RGNNV. • Reaction can be finished at 37 °C in 20 min; time can be further reduced to 5 min for high viral load sample. • The […]
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SERS-Based Lateral Flow Strip Biosensor for Simultaneous Detection of Listeria monocytogenes and Salmonella enterica Serotype Enteritidis
Author: Liu HB, Du XJ, Zang YX, Li P, Wang S.We developed a new surface-enhanced Raman scattering (SERS)-based lateral flow (LF) strip biosensor combined with recombinase polymerase amplification (RPA) for simultaneous detection of Listeria monocytogenes and Salmonella enterica serotype Enteritidis. AuMBA@Ag core–shell nanoparticles were used in this SERS-LF.
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Development and evaluation of Loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples.
Author: Garrido-Maestu A, Azinheiro S, Carvalho J, Fucinos P, Prado M.•LAMP and RPA methods were developed for the detection of L. monocytogenes. •Performance of the new methods was compared against two reference real-time PCR methods. •A very low limit of detection, with high confidence, was obtained for both methodologies. •Both […]
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Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for detection of Toxoplasma gondii in the environment
Author: Wu YD, Xu MJ, Wang QQ, Zhou CX, Wang M, Zhu XQ, Zhou DH.The amplification product was visualized by the lateral flow (LF) strip within 5 min using the specific probe added to the RPA reaction system. The sensitivity of the established assay was 10 times higher than that of nested PCR with a […]
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Development of field-applicable tests for rapid and sensitive detection of Candidatus Phytoplasma oryzae
Author: Wambua L, Schneider B, Okwaro A, Wanga JO, Imali O, Wambua PN, Agutu L, Olds C, Jones CS, Masiga D, Midega C, Khan Z, Jores J, Fischer A.The assay is based on the amplification of an imp gene fragment, highly specific for this pathogen. Moreover, we demonstrated that this assay can be used on phloem sap directly squeezed from infected plant material and that the pathogen, can be detected […]
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A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay.
Author: Babu B, Washburn BK, Ertekc TS, Miller SH, Riddlea CB, Knox GW, Ochoa-Corona FM, Olsond J, Katırcıoğlue YZ.RT-exo RPA analysis of the infected plants using the primer/probe indicated that the virus could be detected from leaves, stems, petals, pollen, primary roots and secondary roots.
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Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors
Author: Hu C, Kalsi S, Zeimpekis I, Sun K, Ashburn P, Turner C, Sutton JM, Morgan H.A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method.
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Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Common Bacterial Urinary Tract Infection Pathogens
Author: Raja B, Goux HJ, Marapadaga A, Rajagopalan S, Kourentzi K, Willson RC.The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterococcus faecalis. All five RPAs required reaction times of under 12 minutes to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with […]
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Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars
Author: Kapoor R, Srivastava N, Kumar S, Saritha RK, Sharma SK, Jain RK, Baranwal VK.In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both […]