Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification
Author: Wang J, Wang J, Li R, Liu L, Yuan W.The RT-RPA assay was performed successfully at 40 °C, and the results were obtained within 3 min–12 min. The assay could detect CDV, but did not show cross-detection of canine parvovirus-2 (CPV-2), canine coronavirus (CCoV), canine parainfluenza virus (CPIV), pseudorabies virus (PRV) or […]
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Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus.
Author: Yang Y, Qin X, Zhang X, Zhao Z, Zhang W, Zhu X, Cong G, Li Y, Zhang Z.Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively.
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Development of an isothermal amplification-based assay for the rapid visual detection of Salmonella bacteria.
Author: Liu HB, Zang YX, Du XJ, Li P, Wang S.Importantly, Salmonella could be detected in milk and chicken breast at concentrations as low as 1.05 × 100 cfu/mL or 1.05 × 100 cfu/g after enrichment for 2 h and in eggs at 1.05 × 100 cfu/g after enrichment for 4 h. Furthermore, RPA was […]
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An exo probe-based recombinase polymerase amplification assay for the rapid detection of porcine parvovirus
Author: Wang JC, Liu LB, Han QA , Wang JF, Yuan WZRecombinase polymerase amplification (RPA), an isothermal amplification technology, has been developed as an alternative to PCR in pathogen detection.
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Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for detection of Toxoplasma gondii in the environment
Author: Wu YD, Xu MJ, Wang QQ, Zhou CX, Wang M, Zhu XQ, Zhou DH.The amplification product was visualized by the lateral flow (LF) strip within 5 min using the specific probe added to the RPA reaction system. The sensitivity of the established assay was 10 times higher than that of nested PCR with a […]
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A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay.
Author: Babu B, Washburn BK, Ertekc TS, Miller SH, Riddlea CB, Knox GW, Ochoa-Corona FM, Olsond J, Katırcıoğlue YZ.RT-exo RPA analysis of the infected plants using the primer/probe indicated that the virus could be detected from leaves, stems, petals, pollen, primary roots and secondary roots.
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Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors
Author: Hu C, Kalsi S, Zeimpekis I, Sun K, Ashburn P, Turner C, Sutton JM, Morgan H.A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method.
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Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Common Bacterial Urinary Tract Infection Pathogens
Author: Raja B, Goux HJ, Marapadaga A, Rajagopalan S, Kourentzi K, Willson RC.The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterococcus faecalis. All five RPAs required reaction times of under 12 minutes to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with […]
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Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars
Author: Kapoor R, Srivastava N, Kumar S, Saritha RK, Sharma SK, Jain RK, Baranwal VK.In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both […]
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Development of Real-Time Isothermal Amplification Assays for On-Site Detection of Phytophthora infestans in Potato Leaves
Author: Ammour MS, Bilodeau GJ, Tremblay DM, Van der Heyden H, Yaseen T, Varvaro L, Carisse O.The assay’s specificity was tested using several species of Phytophthora and other potato fungal and oomycete pathogens. Both LAMP and RPA assays showed specificity to P. infestans but also to the closely related species P. andina, P. mirabilis, P. phaseoli, and P. ipomoeae, although the latter are not […]