Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors
Author: Hu C, Kalsi S, Zeimpekis I, Sun K, Ashburn P, Turner C, Sutton JM, Morgan H.A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method.
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Development of an isothermal amplification-based assay for the rapid visual detection of Salmonella bacteria.
Author: Liu HB, Zang YX, Du XJ, Li P, Wang S.Importantly, Salmonella could be detected in milk and chicken breast at concentrations as low as 1.05 × 100 cfu/mL or 1.05 × 100 cfu/g after enrichment for 2 h and in eggs at 1.05 × 100 cfu/g after enrichment for 4 h. Furthermore, RPA was […]
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Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for detection of Toxoplasma gondii in the environment
Author: Wu YD, Xu MJ, Wang QQ, Zhou CX, Wang M, Zhu XQ, Zhou DH.The amplification product was visualized by the lateral flow (LF) strip within 5 min using the specific probe added to the RPA reaction system. The sensitivity of the established assay was 10 times higher than that of nested PCR with a […]
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Development of field-applicable tests for rapid and sensitive detection of Candidatus Phytoplasma oryzae
Author: Wambua L, Schneider B, Okwaro A, Wanga JO, Imali O, Wambua PN, Agutu L, Olds C, Jones CS, Masiga D, Midega C, Khan Z, Jores J, Fischer A.The assay is based on the amplification of an imp gene fragment, highly specific for this pathogen. Moreover, we demonstrated that this assay can be used on phloem sap directly squeezed from infected plant material and that the pathogen, can be detected […]
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Enhanced solid-phase recombinase polymerase amplification and electrochemical detection
Author: Jauset-Rubio M, Lobato IM, Mayboroda O, Katakis I, O’Sullivan CK.Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio (1:0, 1:1, 1:10 and 1:100) and length […]
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Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection
Author: Yamanaka ES, Tortajada-Genaro LA, Maquieira Á.An amplification chip platform containing 100 wells was manufactured with a 3D printer and using thermoplastic polylactic acid. The platform reduces reagent consumption and allows parallelization.
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Nucleic acid detection with CRISPR-Cas13a/C2c2
Author: Gootenberg JS, Abudayyeh OO, Wook Lee J, Essletzbichler P, Dy AJ, Joung J, Verdine V, Zhang F, et alWe use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumour DNA.
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Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus
Author: Yang Y, Qin X, Zhang W, Li Z, Zhang S, Li Y, Zhang Z.Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV).
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Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip
Author: Yeh EC, Fu C-C, Hu L, Thakur R, Feng J, Lee LP.We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps.
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Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification
Author: Wang R, Zhang F, Wang L, Qian W, Qian C, Wu J, Ying Y.In this paper, a simple, visual and instrument-free method for instant on-site detection of GTS 40-3-2 soybean has been developed. It is based on body-heat recombinase polymerase amplification (RPA) and followed with naked-eye detection via fluorescent DNA dye.