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Reverse Transcription–Recombinase Polymerase Amplification Assay for the Detection of Sugarcane Streak Mosaic Virus in Sugarcane.Author: Feng XY, Shen LB, Wang WZ, Wang JG, Cao ZY, Zhang SZ.
In this study, a novel isothermal assay was established on the basis of reverse transcription–recombinase polymerase amplification (RT-RPA) and evaluated in terms of its ability to detect SCSMV. Overall, the RT-RPA assay was specific, sensitive, reliable, and rapid for SCSMV …
Recombinase polymerase amplification assay for rapid detection of the root-knot nematode Meloidogyne enterolobii.Author: Subbotin SA.
RPA assays were developed targeting the IGS rRNA gene of the pacara earpod tree root-knot nematode, Meloidogyne enterolobii. The RPA assays using TwistAmp® Basic and TwistAmp® exo kits allowed detection of M. enterolobii from gall tissues and crude nematode extracts …
Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1.Author: Wang H, Hou P, Zhao G, Yu L, Gao Y, He H.
Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV) is one of the most highly infectious diseases in livestock, and leads to huge economic losses. In this study, a series of serotype-specific reverse transcription recombinase polymerase amplification assays combined with …
Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1. Read More
Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.Author: Sun N, Wang W, Wang J, Yao X, Chen F, Li X, Yinglei Y, Chen B.
Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. The sensitivity of RT-RPA-LFD assays is …
High-specificity Double-stranded DNA Detection with “Humanoid” Molecular Beacon and TALEs.Author: Wang Y, Bai LJ, Qu X, Gao Y, Wang J, Li S, Fan L, Wei H, Liu S, Peng Y, Gao Z, Zhu Y, Gao Z, Ning B.
Owing to the limitation of DNA-specific recognition, improving the sensitivity of bacteria detection without additional culture poses a considerable challenge. Herein, a sensitive, novel, and high-specificity fluorescence detection method was developed using transcription activator-like effectors (TALEs) as the specifically recognized …
Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plantsAuthor: Zhao C, Sun F, Li X, Lan Y, Du L, Zhou T, Zhou Y.
Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallén), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method …
Rapid detection of cyanobacteria by recombinase polymerase amplification combined with lateral flow strips.Author: Li J, Wang C, Yu X, Lin H, Hui C, Shuai L, Zhang S.
Cyanobacteria are one of the major groups of algae causing algal blooms. In this study a rapid method for detecting Cyanobacteria was developed using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. The method developed in …
Capture, Concentration and Detection of Salmonella in Foods Using Magnetic Ionic Liquids and Recombinase Polymerase Amplification.Author: Hice SA, Clark KD, Anderson JL, Brehm-Stecher BF.
This study combines magnetic ionic liquid (MIL)-based sample preparation with isothermal amplification and detection of Salmonella-specific DNA using Recombinase Polymerase Amplification (RPA).
A Sample-to-Targeted Gene Analysis Biochip for Nanofluidic Manipulation of Solid-Phase Circulating Tumor Nucleic Acid Amplification in Liquid Biopsies.Author: Koo KM, Dey S, Trau M.
In this study, complex liquid biopsy sample-to-targeted genetic analysis was conducted on a biochip with a 50 copies-detection limit within 30 min. The biochip uniquely integrated electrical lysis and release of cellular targets with minimal processing; nanofluidic manipulation to accelerate …
Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.Author: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M.
Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. …