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High-specificity Double-stranded DNA Detection with “Humanoid” Molecular Beacon and TALEs.Author: Wang Y, Bai LJ, Qu X, Gao Y, Wang J, Li S, Fan L, Wei H, Liu S, Peng Y, Gao Z, Zhu Y, Gao Z, Ning B.
Owing to the limitation of DNA-specific recognition, improving the sensitivity of bacteria detection without additional culture poses a considerable challenge. Herein, a sensitive, novel, and high-specificity fluorescence detection method was developed using transcription activator-like effectors (TALEs) as the specifically recognized …
Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plantsAuthor: Zhao C, Sun F, Li X, Lan Y, Du L, Zhou T, Zhou Y.
Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallén), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method …
Duplex lateral flow assay for the simultaneous detection of Yersinia pestis and Francisella tularensis.Author: Jauset-Rubio M, Tomaso H, Soror El-Shahawi M, Saleh Omar Bashammakh A, Obaid Al-Youbi A, O' Sullivan CK.
The multiplexed amplification detection of genomic DNA from Francisella tularensis and Yersinia pestis is reported here. Amplification was achieved using isothermal recombinase polymerase amplification (RPA), exploiting tailed primers, followed by detection using a nucleic-acid lateral flow assay.
Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.Author: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M.
Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. …
Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil.Author: Burkhardt A, Ramon ML, Smith B, Koike ST, Martin FN.
RPA has been validated on over 200 infected strawberry plants with a diagnostic sensitivity of 93% and a diagnostic specificity of 99%, respectively. Together, this work demonstrates the value of using new approaches to identify loci for detection and provides …
PrimedRPA: Primer design for Recombinase polymerase amplification assays.Author: Higgins M, Ravenhall M, Ward D, Phelan J, Ibrahim A, Forrest MS, Clark TG, Campino S.
PrimedRPA is a python-based package that automates the creation and filtering of RPA primers and probe sets. It aligns several sequences to identify conserved targets, and filters regions that cross react with possible background organisms.
A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.Author: Zhao G, Wang H, Hou P, Xia X, He H.
In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA).
Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays.Author: Singpanomchai N, Akeda Y, Tomono K, Tamaru A, Santanirand P, Ratthawongjirakul P.
Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, …
Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses.Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.
The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples.
Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay.Author: Toldra A, Jauset-Rubio M, Andree KB, Fernandez-Tejedor M, Diogene J, Katakis I, O'Sullivan CK, Campas M.
An assay for the detection of two Karlodinium species has been developed. The assay is based on isothermal DNA amplification and a sandwich hybridisation. The assay discriminates between Karlodinium veneficum and Karlodinium armiger. Seawater samples spiked with Karlodinium cells have …