Publications

Publications

Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification.

    Author: Ma S, Li X, Peng B, Wu W, Wang X, Liu H, Yuan L, Fang S, Lu J.

    In this study, a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9 was developed and evaluated. The H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic …

    Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification. Read More

  • Single-Step Recombinase Polymerase Amplification Assay Based on a Paper Chip for Simultaneous Detection of Multiple Foodborne Pathogens.

    Author: Ahn H, Batule BS, Seok Y, Kim MG.

    A paper chip device-based recombinase polymerase amplification (RPA) method was developed for highly sensitive and selective single-step detection of foodborne pathogens. The diagnostic utility of the device was demonstrated by the reliable detection of E. coli and S.aureus present in …

    Single-Step Recombinase Polymerase Amplification Assay Based on a Paper Chip for Simultaneous Detection of Multiple Foodborne Pathogens. Read More

  • Rapid detection of foot-and-mouth disease virus using reverse transcription recombinase polymerase amplification combined with a lateral flow dipstick.

    Author: Wang H, Zhao G, Hou P, Yu L, He C, He H.

    The RPA-LFD assay was highly sensitive, detecting down to 10 copies of plasmid DNA. There was no cross-reactivity with other pathogens causing vesicular lesions. In addition, 143 clinical samples were used to compare RPA-LFD with real-time PCR, with 98.6% concordance …

    Rapid detection of foot-and-mouth disease virus using reverse transcription recombinase polymerase amplification combined with a lateral flow dipstick. Read More

  • Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil.

    Author: Burkhardt A, Ramon ML, Smith B, Koike ST, Martin FN.

    RPA has been validated on over 200 infected strawberry plants with a diagnostic sensitivity of 93% and a diagnostic specificity of 99%, respectively. Together, this work demonstrates the value of using new approaches to identify loci for detection and provides …

    Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil. Read More

  • Recombinase Polymerase Amplification Combined with Unmodified Gold Nanoparticles for Salmonella Detection in Milk.

    Author: Chen ZG, Zhong HX, Luo H, Zhang RY, Huang JR.

    In this study, isothermal recombinase polymerase amplification (RPA) was combined with unmodified gold nanoparticles (AuNPs) to detect Salmonella in milk. A rapid, effective, visualized, and low-cost RPA-AuNP assay was developed, with a detection limit of 50 CFU for milk samples …

    Recombinase Polymerase Amplification Combined with Unmodified Gold Nanoparticles for Salmonella Detection in Milk. Read More

  • BioBits™ Explorer: A modular synthetic biology education kit.

    Author: Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ.

    BioBits™ Explorer kit enables hands-on demonstrations of cutting-edge science that are inexpensive and easy to use, circumventing many current barriers for implementing exploratory biology experiments in classrooms.

  • A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids.

    Author: Yang H, Chen Z, Cao X, Li Z, Stavrakis S, Choo J, deMello AJ, Howes PD, He N.

    An integrated sample-in-digital-answer-out (SIDAO) diagnostic system incorporating DNA extraction and digital recombinase polymerase amplification, which enables rapid and quantitative nucleic acid analysis from bodily fluids within a disposable cartridge.

  • Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses.

    Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.

    The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples.

  • Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler’s murine encephalomyelitis virus.

    Author: Ma L, Cong F, Zhu Y, Wu M, Xu F, Huang R, Moore RJ, Guo P.

    A reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. The sensitivity of the RT-RPA assay approached 8 copies per reaction, which is equivalent to the sensitivity of RT-qPCR reactions.

  • Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays.

    Author: Singpanomchai N, Akeda Y, Tomono K, Tamaru A, Santanirand P, Ratthawongjirakul P.

    Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, …

    Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays. Read More

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