Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus.
Author: Kumar PV, Sharma SK, Rishi N, Ghosh DK, Baranwal VK.The results from the present study indicated that RPA assay can be used easily in routine indexing of citrus planting material. To the best of our knowledge, this is the first report on development of a rapid and simplified isothermal […]
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Simple and label-free pathogen enrichment via homobifunctional imidoesters using a microfluidic (SLIM) system for ultrasensitive pathogen detection in various clinical specimens
Author: Jin CE, Koo B, Lee EY, Kim JY, Kim SH, Shin Y.We demonstrated its clinical utility in large sample volumes from 46 clinical specimens including environmental swabs, saliva, and blood plasma. The SLIM system showed higher sensitivity with these samples and could detect pathogens that were below the threshold of detection […]
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Detection of Entamoeba Species: A Comparative Analysis of Nested Multiplex PCR and Recombinase Polymerase Amplification
Author: Philips SA, Manochitra K, Padukone S, Chandra S. Parija.The aim of the present study was to compare the diagnostic ability of nested-multiplex PCR and Recombinase polymerase amplification for differential detection of E. histolytica and E. dispar. The results of this study showed good agreement between the two tests. […]
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Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus
Author: Tan KK, Azizan NS, Yaacob CN, Che Mat Seri NAA, Samsudin NI, Teoh BT, Sam SS, AbuBakar S.The dengue RT-RPA assay can be successfully performed by simply following the provided written instructions. Deviations from the written protocols did not adversely affect the outcome of the assay. These suggest that the RT-RPA assay is indeed a simple, robust […]
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Sensitive and specific recombinase polymerase amplification set of assays for fast screening, detection and identification of Bacillus anthracis in a field setting.
Author: Bentahir M, Ambroise J, Delcorps C, Pilo P, Gala JL.RPA tests displayed 100% specificity and sensitivity. The hands-off turnaround time at 42°C ranged from 5 to 6 min for 102 genomic copies. The analytical sensitivity of each RPA was ∼10 molecules per reaction. Besides, BA_5345 and adk RPA assays […]
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Application of recombinase polymerase amplification in the detection of Pseudomonas aeruginosa
Author: Jin XJ, Gong YL, Yang L, Mo BH, Peng YZ, He P, Zhao JN, Li XL.The detection limit of Pseudomonas aeruginosa in real-time PCR and PCR was 1×10 7 CFU/mL, and the detection limit of Pseudomonas aeruginosa in RPA was 1×10 2 CFU/mL.RPA. In fluorescence quantitative PCR, the higher the concentration of Pseudomonas aeruginosa, the […]
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Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future
Author: James AS, Todd S, Pollak NM, Marsh GA, Macdonald J.The assay had a detection limit of 134 copies per μL of plasmid containing the NP gene of Ebolavirus Mayinga, and cultured Ebolavirus and was highly specific for the Zaire ebolavirus species, including the Guinea strain responsible for the 2014/2015 […]
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A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia
Author: Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M.The analytical sensitivity in the multiplex assay amplifying specific regions of S. pneumoniae and L. pneumophila simultaneously was 10 CFUs of genomic DNA per reaction. In cross detection studies with closely related strains and other bacterial agents the specificity of the […]
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Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2
Author: Liu L, Wang J, Geng Y, Wang J, Li R, Shi R, Yuan W.The assay performance was further evaluated by testing 60 canine fecal samples, and CPV-2 DNA was detected in 46 samples (76.7%, 46/60) by LFS RPA, which was the same result as that of the real-time PCR assay and higher than […]
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Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids
Author: Soliman H, Kumar G, El-Matbouli M.A novel PKD-RPA assay for the detection of T. bryosalmonae was developed. The assay offers considerable advantages including speed, sensitivity, specificity and visual detection. Applying the PKD-RPA assay combined with an LFD enhances the surveillance and early detection of T. […]