Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
-
Application of recombinase polymerase amplification in the detection of Pseudomonas aeruginosa
Author: Jin XJ, Gong YL, Yang L, Mo BH, Peng YZ, He P, Zhao JN, Li XL.The detection limit of Pseudomonas aeruginosa in real-time PCR and PCR was 1×10 7 CFU/mL, and the detection limit of Pseudomonas aeruginosa in RPA was 1×10 2 CFU/mL.RPA. In fluorescence quantitative PCR, the higher the concentration of Pseudomonas aeruginosa, the […]
-
An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus.
Author: Kumar PV, Sharma SK, Rishi N, Ghosh DK, Baranwal VK.The results from the present study indicated that RPA assay can be used easily in routine indexing of citrus planting material. To the best of our knowledge, this is the first report on development of a rapid and simplified isothermal […]
-
Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Listeria monocytogenes Detection in Food.
Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S.Experiments confirmed a detection limit as low as 300 fg of DNA and 1.5 × 101 CFU in pure cultures. Furthermore, RPA‐LF exhibited no cross‐reactions with pathogens. Evaluation of the method with food samples indicated that the detection limit was […]
-
Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus
Author: Tan KK, Azizan NS, Yaacob CN, Che Mat Seri NAA, Samsudin NI, Teoh BT, Sam SS, AbuBakar S.The dengue RT-RPA assay can be successfully performed by simply following the provided written instructions. Deviations from the written protocols did not adversely affect the outcome of the assay. These suggest that the RT-RPA assay is indeed a simple, robust […]
-
Development of recombinase polymerase amplification assays for the rapid detection of peste des petits ruminants virus
Author: Zhang Y, Wang J, Zhang Z, Mei L, Wang J, Wu S, Lin X.Clinical evaluation using 162 ovine and hircine serum and nasal swab samples showed that the performance of both the real-time RT-RPA assay and the conventional RT-RPA assay were comparable to that of real-time RT-PCR.
-
Rapid detection of potyviruses from crude plant extracts
Author: Silva G, Oyekanmi J, Nkere CK, Bomer M, Kumar L, Seal SE.The direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be […]
-
Duplex recombinase polymerase amplification assays incorporating competitive internal controls for bacterial meningitis detection.
Author: Higgins O, Clancy E, Forrest MS, Piepenburg O, Cormican M, Boo TW, McGuinness C, O'Sullivan N, Cafferty D, Cunney R, Smith TJ.Clinical performance of each internally controlled duplex RPA assay was evaluated by testing 64 archived PCR-positive clinical samples. Compared to real-time PCR, all duplex RPA assays demonstrated 100% diagnostic specificity, with diagnostic sensitivities of 100%, 86.3% and 100% for the […]
-
Molecular Detection of the Theileria Annulata in Cattle from different regions of Punjab, Pakistan using Recombinase Polymerase Amplification and PCR
Author: Hassan MA, Liu J, Sajid MS, Mahmood A, Zhao SY, Abbas Q, Guan G, Yin H, Luo J.All 274 samples were screened using conventional PCR and 21 (7.66%) samples were positive for T. annulata. All the samples that were RPA positive but PCR negative were sequenced, which confirmed the results of RPA. The highest positive rate was […]
-
Development of a recombinase polymerase amplification assay for Vibrio parahaemolyticus detection with an internal amplification control.
Author: Yang H, Wei S, Gooneratne R, Mutukumirad AN, Ma X, Tang S, Wu X.The sensitivity of the assay was determined as 3×103 CFU/mL, which is decidedly more sensitive than the established PCR method. This method was then used to test seafood samples which were collected from local market. 7 out of 53 different […]
-
Recombinase polymerase amplification combined with lateral flow dipstick for equipment-free detection of Salmonella in shellfish.
Author: Gao W, Huang H, Zhu P, Yan X, Fan J, Jiang J, Xu J.The RPA-LFD was able to function at 30-45 °C, and at the temperature of 40 °C, it only took 8 min of amplification to reach the test threshold of amplicons. The established method had both a good specificity and a […]