Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Design and Clinical Verification of Surface Enhanced Raman Spectroscopy Diagnostic Technology for Individual Cancer Risk Prediction.
Author: Koo KM, Wang J, Richards RS, Farrell A, Yaxley JW, Samaratunga H, Teloken PE, Roberts MJ, Coughlin GD, Lavin MF, Mainwaring PN, Wang Y, Gardiner RA, Trau M.Describes a comprehensive clinical verification of a prospective label-free surface-enhanced Raman scattering (SERS) nanodiagnostic assay for prostate cancer (PCa) risk stratification.
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Akkermansia muciniphila as a model case for the development of an improved quantitative RPA microbiome assay.
Author: Goux H, Chavan D, Crum M, Kourentzi K, Willson RC.Removing DNA with anti-DNA antibodies enabled the development of a quantitative RPA microbiome assay capable of determining the relative abundance of the physiologically-important bacterium Akkermansia muciniphila in human feces.
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Semi-quantitative nucleic acid test with simultaneous isotachophoretic extraction and amplification.
Author: Bender AT, Borysiak MD, Levenson AM, Lillis L, Boyle DS, Posner JD.We demonstrate simultaneous ITP and RPA can consistently detect 5 copies per reaction in buffer and 10 000 copies per milliliter of human serum with no intermediate user steps. We also show preliminary extraction and amplification of DNA from whole blood […]
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Fast and sensitive isothermal DNA assay using micro-bead dielectrophoresis for detection of anti-microbial resistance genes.
Author: Nakano M, Kalsi S, Morgan H.Fast and sensitive detection of anti-microbial resistance gene. Combination of isothermal DNA amplification and microbeads-based dielectrophoretic impedance measurement. Amplicon of target gene was attached on microbeads, then, the amplicon-labeled microbeads were detected. Simultaneous amplification and labeling method was proposed. Detection […]
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Isothermal amplification using modified primers for rapid electrochemical analysis of coeliac disease associated DQB1*02 HLA allele.
Author: Al-Madhagi S, Joda H, Jauset-Rubio M, Ortiz M, Katakis I, O'Sullivan CK.The genosensor was applied to genomic DNA and real patient and control blood samples for detection of the coeliac disease associated DQB1*02 HLA allele, as a model system, demonstrating the possibility to carry out molecular diagnostics, combining amplification and detection […]
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A novel method to detect meat adulteration by recombinase polymerase amplification and SYBR green I
Author: Cao Y, Zheng K, Jiang J, Wu J, Shi F, Song X, Jiang Y.At the isothermal temperature of 37 °C, RPA specifically identifies duck, chicken, cow, sheep and pig within 30 min of water bath. The RPA amplicons were successfully visualized by adding SG I. Furthermore, RPA can differentiate species of boiled, microwaved, high pressured […]
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Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method.
Author: Wand NIV, Bonney LC, Watson RJ, Graham V, Hewson R.A novel ZIKV real-time reverse transcriptase RPA (RT-RPA) assay capable of detecting a range of different ZIKV strains from a variety of geographical locations. The ZIKV RT-RPA was shown to be highly sensitive, being capable of detecting as few as […]
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A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses
Author: Tian AL, Elsheikha HM, Zhou DH, Wu YD, Chen MX, Wang M, Chen D, Zhang XC, Zhu XQ.A recombinase polymerase amplification (RPA) assay combined with lateral flow (LF) strips was developed for the detection of N. caninum. The LF-RPA assay distinguished N. caninum from nine closely related protozoan species. The amplification reaction was completed in about 10 min […]
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Efficacy of Recombinase Polymerase Amplification to Diagnose Trypanosoma cruzi Infection in Dogs with Cardiac Alterations from an Endemic Area of Mexico
Author: Jimenez-Coello M, Shelite T, Castellanos-Gonzalez A, Saldarriaga O, Rivero R, Ortega-Pacheco A, Garg N, Acevedo-Arcique C, Amaya-Guardia K, Melby P, Travi BL.The analytical sensitivity indicated that RPA-LF amplified T. cruzi DNA in samples containing almost equal to one to two parasites per reaction. Serial twofold dilutions of T. cruzi epimastigotes showed that the test had 95% (19/20) repeatability at concentrations of […]
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Field Detection of Citrus Huanglongbing Associated with ‘Candidatus Liberibacter Asiaticus’ by Recombinese Polymerase Amplification within 15 min.
Author: Qian W, Lu Y, Meng Y, Ye Z, Wang L, Wang R, Zheng Q, Wu J.In this paper, an extremely rapid and simple method for field detection of Las from leaf samples, based on recombinase polymerase amplification (RPA), is described. Three RPA primer pairs were designed and evaluated. RPA amplification was optimized so that it […]