Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification.
Author: Hammond RW, Zhang S.A molecular diagnostic assay utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 39 °C and target-specific primers and probe were developed for the rapid, sensitive, and specific detection of tomato chlorotic dwarf viroid (TCDVd) in infected […]
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A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.
Author: Kevin M. Koo, Eugene J. H. Wee, Paul N. Mainwaring & Matt TrauWe describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment.
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Enhanced solid-phase recombinase polymerase amplification and electrochemical detection
Author: Jauset-Rubio M, Lobato IM, Mayboroda O, Katakis I, O’Sullivan CK.Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio (1:0, 1:1, 1:10 and 1:100) and length […]
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Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection
Author: Yamanaka ES, Tortajada-Genaro LA, Maquieira Á.An amplification chip platform containing 100 wells was manufactured with a 3D printer and using thermoplastic polylactic acid. The platform reduces reagent consumption and allows parallelization.
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Aboriginal mitogenomes reveal 50,000 years of regionalism in Australia
Author: Tobler R, Rohrlach A, Soubrier J, Bover P, Llamas B, Tuke J, Bean N, Cooper A et alHere we report 111 mitochondrial genomes (mitogenomes) from historical Aboriginal Australian hair samples, whose origins enable us to reconstruct Australian phylogeographic history before European settlement.
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Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification
Author: Wang R, Zhang F, Wang L, Qian W, Qian C, Wu J, Ying Y.In this paper, a simple, visual and instrument-free method for instant on-site detection of GTS 40-3-2 soybean has been developed. It is based on body-heat recombinase polymerase amplification (RPA) and followed with naked-eye detection via fluorescent DNA dye.
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Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip
Author: Yeh EC, Fu C-C, Hu L, Thakur R, Feng J, Lee LP.We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps.
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Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus
Author: Yang Y, Qin X, Zhang W, Li Z, Zhang S, Li Y, Zhang Z.Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV).
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Nucleic acid detection with CRISPR-Cas13a/C2c2
Author: Gootenberg JS, Abudayyeh OO, Wook Lee J, Essletzbichler P, Dy AJ, Joung J, Verdine V, Zhang F, et alWe use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumour DNA.
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Development of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection
Author: Tu PA, Shiu JS, Lee SH, Pang VF, Wang DC, Wang PH.Under the optimal incubation conditions, specifically, 30 min at 37 °C for RPA followed by 5 min at room temperature for LFD, the assay was found to be sensitive to a lower limit of 80 pg of total DNA and […]