Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Electrochemical detection of Francisella tularensis genomic DNA using solid-phase recombinase polymerase amplification
Author: Jonathan Sabaté del Río, Nouran Yehia Adly, Josep Lluis Acero-Sánchez, Olivier Y.F. Henry, Ciara K. O'SullivanSolid-phase isothermal DNA amplification was performed exploiting the homology protein recombinase A (recA). The system was primarily tested on maleimide activated microtitre plates as a proof-of-concept and later translated to an electrochemical platform.
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Isothermal Method of a Recombinase Polymerase Amplification Assay for the Detection of Most Common High-Risk Human Papillomavirus Type 16 and Type 18 DNA
Author: Biao Ma, Jiehong Fang, Mingzhou Zhang et alIn this study, we evaluate the efficacy of the RPA assay, incubating clinical specimens of HPV16 and HPV18 using plasmids standard. It operates at constant low temperature without the thermal instrumentation for incubation.
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Development of real-time and lateral flow strip reverse transcription recombinase polymerase amplification assays for rapid detection of peste des petits ruminants virus
Author: Yang Yang, Xiaodong Qi, Zhidong Zhang et alIn this study, reverse transcription recombinase polymerase amplification assays using real-time fluorescent detection (real-time RT-RPA assay) and lateral flow strip detection (LFS RT-RPA assay) were developed targeting the N gene of PPRV.
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Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay
Author: Liyuan Liu, Luzhi Jiang, Yongjie Wang et alA rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C
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Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions
Author: Fangyuan Yin, Junlong Liu, Aihong Liu, Youquan Li, Jianxun Luo, Guiquan GuanHerein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples.
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Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China
Author: Weiwei Xing, Xinling Yu, Donggang Xu et alThe S.japonicum RPA assay was developed to target highly repetitive retrotransposon SjR2 gene of S japonicum, and its sensitivity and specificity were assessed by serial dilution of S. japonicum genomic DNA and other related worm genomic DNA respectively.
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A nanoplasmonic label-free surface-enhanced Raman scattering strategy for non-invasive cancer genetic subtyping in patient samples
Author: Jing Wang, Kevin M. Koo, Eugene J. H. Wee, Yuling Wang, Matt TrauWe herein present a novel strategy based on multiplex reverse transcription-recombinase polymerase amplification (RT-RPA) to enrich multiple RNA biomarkers, followed by label-free SERS with multivariate statistical analysis to directly detect, identify and distinguish between these long amplicons (∼200 bp).
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Inhibition of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA
Author: Brittany Rohrman and Rebecca Richards-KortumIn this paper, we show that the concentrations of background DNA found in whole blood prevent the amplification of target DNA by RPA. First, using an HIV-1 RPA assay with known concentrations of nonspecific background DNA, we show that RPA […]
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Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification
Author: Jaroenram W. and Owens L.The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis.
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Development of Rapid Isothermal Amplification Assays for Detection of Phytophthora spp. in Plant Tissue
Author: Miles TD, Martin FN, Coffey MD.The lower limit of linear detection using purified DNA was 200 to 300fg of DNA in all pathogen RPA assays. Six different extraction buffers were tested for use during plant tissue maceration and the assays were validated in the field […]