Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions
Author: Fangyuan Yin, Junlong Liu, Aihong Liu, Youquan Li, Jianxun Luo, Guiquan GuanHerein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples.
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Isothermal Method of a Recombinase Polymerase Amplification Assay for the Detection of Most Common High-Risk Human Papillomavirus Type 16 and Type 18 DNA
Author: Biao Ma, Jiehong Fang, Mingzhou Zhang et alIn this study, we evaluate the efficacy of the RPA assay, incubating clinical specimens of HPV16 and HPV18 using plasmids standard. It operates at constant low temperature without the thermal instrumentation for incubation.
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Development of real-time and lateral flow strip reverse transcription recombinase polymerase amplification assays for rapid detection of peste des petits ruminants virus
Author: Yang Yang, Xiaodong Qi, Zhidong Zhang et alIn this study, reverse transcription recombinase polymerase amplification assays using real-time fluorescent detection (real-time RT-RPA assay) and lateral flow strip detection (LFS RT-RPA assay) were developed targeting the N gene of PPRV.
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Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay
Author: Liyuan Liu, Luzhi Jiang, Yongjie Wang et alA rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C
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A rapid assay for detection of Rose rosette virus using reverse transcription-recombinase polymerase amplification using multiple gene targets
Author: Binoy Babu, Brian K. Washburn, Steven H. Miller, Mathews L. Paret et al• Designed multiple RT-RPA primer sets for Rose rosette virus (RRV). • RT-RPA assay for detection of RRV was developed. • The RPA primer sets were highly specific to RRV. • Primer sets were highly sensitive, detecting up to 1 […]
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Assay for Listeria monocytogenes cells in whole blood using isotachophoresis and recombinase polymerase amplification
Author: Charbel Eid, Juan G. SantiagoWe present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA).
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High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine
Author: Kevin M. Koo, Eugene J.H. Wee, Matt Trau• Non-invasive detection of multiple gene fusion biomarkers in patient urine samples. • 60 min assay which is at least five times faster than traditional multiplexed ligase-based assays. • Requires only 30 ng of starting total RNA sample and has […]
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Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool
Author: Miguel M. Cabada, Jose L. Malaga, Alejandro Castellanos-Gonzalez, A. Clinton White Jr. et alWe report on the characterisation of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing […]
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Rapid molecular detection of Zika virus in urine using recombinase polymerase amplification assay
Author: Ahmed Abd El Wahed, Sabri S. Sanabani, Oumar Faye, Manfred Weidmann et alIn this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes).
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Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6
Author: Wang K, Wu Y, Yin D, Tang S, Hu G,He Y.The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity.