Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay
Author: Yang Yang, Xiaodong Qin, Yingjun Sun, Ting Chen, Zhidong ZhangA novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV).
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Development of a multiplex real-time recombinase polymerase amplification (RPA) assay for rapid quantitative detection of Campylobacter coli and jejuni from eggs and chicken products
Author: Kim JY, Lee JL.•Development of a multiplex real-time RPA assay to detect Campylobacter coli & jejuni. •Quantitative detection within a 20 min runtime using the multiplex Rti-RPA assay. •Equivalent or better detection sensitivity than other PCR/LAMP assays. •Fast procedure, fairly simple, and specific […]
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Ultrasensitive and rapid detection of β-conglutin combining aptamers and isothermal recombinase polymerase amplification
Author: Jauset-Rubio M, Sabaté del Río J, Mairal T, Svobodová M, El-Shahawi MS, Bashammakh AS, Alyoubi AO, O’Sullivan CK.An innovative method termed aptamer-recombinase polymerase amplification (Apta-RPA) exploiting the affinity and specificity of a DNA aptamer selected against the anaphylactic β-conglutin allergen termed β-conglutin binding aptamer II (β-CBA II), facilitating ultrasensitive detection via isothermal amplification.
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꿀벌 병원성 바이러스의 정량적 검출을 위한 정량 실시간 재조합효소-중합효소 증폭법의 개발
Author: Min S-H, Wang J-H, Lim S-J, Lee C-W, Yoon B-S.Development of Quantitative Real-time Recombinase Polymerase Amplification (qRT-RPA) Method for Quantitative Detection against Pathogenic Virus in Honeybee
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Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6
Author: Wang K, Wu Y, Yin D, Tang S, Hu G,He Y.The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity.
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Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool
Author: Miguel M. Cabada, Jose L. Malaga, Alejandro Castellanos-Gonzalez, A. Clinton White Jr. et alWe report on the characterisation of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing […]
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High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine
Author: Kevin M. Koo, Eugene J.H. Wee, Matt Trau• Non-invasive detection of multiple gene fusion biomarkers in patient urine samples. • 60 min assay which is at least five times faster than traditional multiplexed ligase-based assays. • Requires only 30 ng of starting total RNA sample and has […]
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Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay
Author: Miriam Jauset-Rubio, Markéta Svobodová, Teresa Mairal, Ciara K. O´Sullivan et alHere we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a […]
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Assay for Listeria monocytogenes cells in whole blood using isotachophoresis and recombinase polymerase amplification
Author: Charbel Eid, Juan G. SantiagoWe present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA).
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A rapid assay for detection of Rose rosette virus using reverse transcription-recombinase polymerase amplification using multiple gene targets
Author: Binoy Babu, Brian K. Washburn, Steven H. Miller, Mathews L. Paret et al• Designed multiple RT-RPA primer sets for Rose rosette virus (RRV). • RT-RPA assay for detection of RRV was developed. • The RPA primer sets were highly specific to RRV. • Primer sets were highly sensitive, detecting up to 1 […]