Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
-
Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6
Author: Wang K, Wu Y, Yin D, Tang S, Hu G,He Y.The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity.
-
꿀벌 병원성 바이러스의 정량적 검출을 위한 정량 실시간 재조합효소-중합효소 증폭법의 개발
Author: Min S-H, Wang J-H, Lim S-J, Lee C-W, Yoon B-S.Development of Quantitative Real-time Recombinase Polymerase Amplification (qRT-RPA) Method for Quantitative Detection against Pathogenic Virus in Honeybee
-
Ultrasensitive and rapid detection of β-conglutin combining aptamers and isothermal recombinase polymerase amplification
Author: Jauset-Rubio M, Sabaté del Río J, Mairal T, Svobodová M, El-Shahawi MS, Bashammakh AS, Alyoubi AO, O’Sullivan CK.An innovative method termed aptamer-recombinase polymerase amplification (Apta-RPA) exploiting the affinity and specificity of a DNA aptamer selected against the anaphylactic β-conglutin allergen termed β-conglutin binding aptamer II (β-CBA II), facilitating ultrasensitive detection via isothermal amplification.
-
Development of a multiplex real-time recombinase polymerase amplification (RPA) assay for rapid quantitative detection of Campylobacter coli and jejuni from eggs and chicken products
Author: Kim JY, Lee JL.•Development of a multiplex real-time RPA assay to detect Campylobacter coli & jejuni. •Quantitative detection within a 20 min runtime using the multiplex Rti-RPA assay. •Equivalent or better detection sensitivity than other PCR/LAMP assays. •Fast procedure, fairly simple, and specific […]
-
Modular development of a prototype POC molecular diagnostic platform for STIs
Author: Manoharanehru Branavan, Ruth E Mackay, Pascal Craw et al•A low cost, optical, POC molecular diagnostic platform. •Sample preparation using a paper membrane. •Isothermal amplification using HDA and RPA.
-
Rapid Point-of-Use DNA Test for Screening of Genuity® Roundup Ready 2 Yield® Soyabean in Seed Sample
Author: Dilip Chandu, Sudakshina Paul, Mathew Parker, Yelena Dudin, Jennifer King-Sitzes, Tim Perez, Don W. Mittanck, Manali Shah, Kevin C. Glenn, and Olaf PiepenburgA highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples applicable in both laboratory and field-type settings.
-
Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples
Author: Jonathan Sabaté del Río, Marketa Svobodova, Paulina Bustos, Pablo Conejeros, Ciara K. O’SullivanElectrochemical detection of solid-phase RPA of the salmon pathogen Piscirickettsia salmonis in salmon genomic DNA. The system was applied to the analysis of eight real salmon samples, and the method was also compared to qPCR analysis, observing an excellent degree […]
-
A Novel Technique to Detect EGFR Mutations in Lung Cancer
Author: Yuanbin Liu, Ting Lei, Zhiyu Liu, Yanbin Kuang, Jianxin Lyu and Qi WangThis study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as […]
-
Mobile suitcase laboratory for rapid detection of Leishmania donovani using RPA assay
Author: Dinesh Mondal, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain, Susanne Böhlken-Fascher, Greg Matlashewski, Axel Kroeger, Piero Olliaro and Ahmed Abd El WahedAn RPA test for Leishmania donovani was successfully tested using a suitcase laboratory in a hospital in Bangladesh. Forty-eight positive and 48 negative samples showed 100% correlation with PCR results.
-
Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle faeces
Author: Yao-Dong Wu, Dong-Hui Zhou, Long-Xian Zhang, Wen-Bin Zheng, Jian-gang Ma, Meng Wang, Xing-Quan Zhu, Min-Jun XuAn RPA test that detects 0.5 oocyst per reaction, with no cross reactivity to other common protozoan species in the intestine of dairy cattle. A simple, rapid, and cost-effective test, with the potential for further development as a diagnostic kit […]