Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine
Author: Valiadi M, Kalsi S, Jones IG, Turner C, Sutton JM, Morgan HA new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 min using a custom, valve-less microfluidic chip.
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Picoliter Well Array Chip-Based Digital RPA for Absolute Quantification of Nucleic Acids
Author: Zhao Li, Yong Liu,Qingquan Wei, Yuanjie Liu,Wenwen Liu,Xuelian Zhang,Yude YudRPA-on-chip processing required less than 30 min, which was a 4-fold decrease compared to dPCR, requiring approximately 2 h. dRPA on the PWA chip provides a simple and highly sensitive method to quantify nucleic acids without thermal cycling or precise […]
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An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
Author: Omar A. Saldarriaga, Alejandro Castellanos-Gonzalez, Renato Porrozzi et alA novel molecular method (RPA-LF) that could be applied in the field because it does not require sophisticated equipment. It is also very sensitive and specific to detect the principal Leishmania species that produce cutaneous leishmaniasis in Latin America.
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FA mathematical model of recombinase polymerase amplification under continuously stirred conditions
Author: Clint Moody, Heather Newell and Hendrik ViljoenRecombinase Polymerase Amplification (RPA), combines this advantage of isothermal PCR with simplicity and rapid amplification. A mathematical model is presented of Recombinase Polymerase Amplification (RPA) and compared to experimental data.
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Modular development of a prototype POC molecular diagnostic platform for STIs
Author: Manoharanehru Branavan, Ruth E Mackay, Pascal Craw et al•A low cost, optical, POC molecular diagnostic platform. •Sample preparation using a paper membrane. •Isothermal amplification using HDA and RPA.
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Rapid Point-of-Use DNA Test for Screening of Genuity® Roundup Ready 2 Yield® Soyabean in Seed Sample
Author: Dilip Chandu, Sudakshina Paul, Mathew Parker, Yelena Dudin, Jennifer King-Sitzes, Tim Perez, Don W. Mittanck, Manali Shah, Kevin C. Glenn, and Olaf PiepenburgA highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples applicable in both laboratory and field-type settings.
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Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples
Author: Jonathan Sabaté del Río, Marketa Svobodova, Paulina Bustos, Pablo Conejeros, Ciara K. O’SullivanElectrochemical detection of solid-phase RPA of the salmon pathogen Piscirickettsia salmonis in salmon genomic DNA. The system was applied to the analysis of eight real salmon samples, and the method was also compared to qPCR analysis, observing an excellent degree […]
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A Novel Technique to Detect EGFR Mutations in Lung Cancer
Author: Yuanbin Liu, Ting Lei, Zhiyu Liu, Yanbin Kuang, Jianxin Lyu and Qi WangThis study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as […]
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Mobile suitcase laboratory for rapid detection of Leishmania donovani using RPA assay
Author: Dinesh Mondal, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain, Susanne Böhlken-Fascher, Greg Matlashewski, Axel Kroeger, Piero Olliaro and Ahmed Abd El WahedAn RPA test for Leishmania donovani was successfully tested using a suitcase laboratory in a hospital in Bangladesh. Forty-eight positive and 48 negative samples showed 100% correlation with PCR results.
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Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle faeces
Author: Yao-Dong Wu, Dong-Hui Zhou, Long-Xian Zhang, Wen-Bin Zheng, Jian-gang Ma, Meng Wang, Xing-Quan Zhu, Min-Jun XuAn RPA test that detects 0.5 oocyst per reaction, with no cross reactivity to other common protozoan species in the intestine of dairy cattle. A simple, rapid, and cost-effective test, with the potential for further development as a diagnostic kit […]