Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Optimizing Illumina next-gen sequencing library preparation for at-biased genomes
Author: Oyola SO, Otto TD, Gu Y, Maslen G, Manske M, Campino S, Turner DJ, Macinnis B, Kwiatkowski DP, Swerdlow HP, Quail MA.Oyola et al compared a number of different polymerases and PCR methods with RPA for generating libraries for sequencing AT-rich genomes. The TwistAmp Basic reactions were used without optimisation, simply following the instructions in the Quick Guide.
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Rapid Detection of HIV-1 Proviral DNA
Author: Boyle DS, Lehman DA, Lillis L, Peterson D, Singhal M, Armes N, Parker M, Piepenburg O, Overbaugh J.Recombinase polymerase amplification (RPA) is a novel technology that is ideal for an HIV-1 diagnostic, as it amplifies target DNA in
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Development of a panel of RPA assays for the detection of biothreat agents
Author: Euler M, Wang Y, Heidenreich D, Patel P, Strohmeier O, Hakenberg S, Niedrig M, Hufert FT, Weidmann M.Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for […]
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A phaseguided passive batch microfluidic mixing chamber for isothermal amplification
Author: Hakenberg S, Hügle M, Weidmann M, Hufert F, Dame G, Urban GA.With a view to developing a rapid pathogen detection system utilizing isothermal nucleic acid amplification, the necessary micro-mixing step is innovatively implemented on a chip. Passive laminar flow mixing of two 6.5 μl batches differing in viscosity is performed within […]
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Multiplex isothermal solid-phase RPA for DNA-based detection of three bacterial pathogens
Author: Sebastian Kersting, Valentina Rausch, Frank F. Bier, Markus von Nickisch-RosenegkSolid-phase RPA to simultaneously amplify and detect Neisseria gonorrhoeae, Salmonella enterica, methicillin-resistant Staphylococcus aureus (MRSA) and a control plasmid in
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Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings
Author: Escadafal C, Faye O, Sall AA, Faye O, Weidmann M, Strohmeier O, von Stetten F, Drexler J, Eberhard M, Niedrig M, Patel PRPA assays were developed to detect Yellow Fever Virus on three different platforms (real-time with or without microfluidic semi-automated system and lateral-flow assay). The assay was able to detect 20 different YFV strains with no cross-reactions with closely related viruses. […]
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A Fully Integrated Lab-on-a-Disc for Nucleic Acid Analysis of Food-borne Pathogens
Author: Tae-Hyeong Kim, Juhee Park, Chi-Ju Kim, and Yoon-Kyoung ChoTwistFlow® Salmonella tests integrated into a centrifugal microfluidic device that performs DNA extraction, amplification and detection onto a single disc. DNA extraction to readout took 30 minutes with sensitivities of 10 CFU/ml of PBS and 100 CFU/ml of milk.
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Rapid detection of Schmallenberg Virus & Bovine Viral Diarrhoea Virus using isothermal amplification
Author: Aebischer A, Wernike K, Hoffmann B, Beer MAmong a wide range of available technologies, high-speed real-time reverse transcriptase quantitative PCR (RT-qPCR) and the two isothermal amplification techniques loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) represent three promising candidates for integration into mobile pen-side tests.
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One-step digital plasma separation for molecular diagnostics
Author: Erh-Chia Yeh and Luke P. LeeFirst demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.
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Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA
Author: Murinda SE, Ibekwe AM, Zulkaffly S, Cruz A, Park S, Razak N, Paudzai FM, Ab Samad L, Baquir K,Muthaiyah K, Santiago B, Rusli A, Balkcom SRPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.