Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
-
RPA combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris
Author: Jaroenram W, Owens LRecombinase Polymerase Amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome.
-
Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification
Author: Zachary Austin Crannell, Brittany Rohrman, and Rebecca Richards-KortumThis paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.
-
Development of a RPA Assay for the Detection of Pathogenic Leptospira
Author: Ahmed Ahmed, Hans van der Linden and Rudy A. HartskeerlLeptospirosis is a difficult to disease to diagnose. Researchers from KIT Biomedical Research have developed an RPA test that can detect
-
Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription- RPA
Author: Tefera A. Mekuriaa, Shulu Zhangb, Kenneth C. EastwellaRT-RPA and lateral flow used to detect the Little cherry virus 2 coat protein gene. RT-RPA was simple, fast, and specific. The test has the sensitivity of RT-PCR and is more cost effective, being capable of detecting the virus from […]
-
Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA
Author: Murinda SE, Ibekwe AM, Zulkaffly S, Cruz A, Park S, Razak N, Paudzai FM, Ab Samad L, Baquir K,Muthaiyah K, Santiago B, Rusli A, Balkcom SRPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.
-
One-step digital plasma separation for molecular diagnostics
Author: Erh-Chia Yeh and Luke P. LeeFirst demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.
-
Rapid detection of Schmallenberg Virus & Bovine Viral Diarrhoea Virus using isothermal amplification
Author: Aebischer A, Wernike K, Hoffmann B, Beer MAmong a wide range of available technologies, high-speed real-time reverse transcriptase quantitative PCR (RT-qPCR) and the two isothermal amplification techniques loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) represent three promising candidates for integration into mobile pen-side tests.
-
A Fully Integrated Lab-on-a-Disc for Nucleic Acid Analysis of Food-borne Pathogens
Author: Tae-Hyeong Kim, Juhee Park, Chi-Ju Kim, and Yoon-Kyoung ChoTwistFlow® Salmonella tests integrated into a centrifugal microfluidic device that performs DNA extraction, amplification and detection onto a single disc. DNA extraction to readout took 30 minutes with sensitivities of 10 CFU/ml of PBS and 100 CFU/ml of milk.
-
Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification
Author: Sebastian Kersting, Valentina Rausch, Frank Fabian Bier, and Markus von Nickisch-RosenegkA TwistAmp nfo-based test for the 18S rRNA gene of P. falciparum detected as few as four parasites after
-
Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings
Author: Escadafal C, Faye O, Sall AA, Faye O, Weidmann M, Strohmeier O, von Stetten F, Drexler J, Eberhard M, Niedrig M, Patel PRPA assays were developed to detect Yellow Fever Virus on three different platforms (real-time with or without microfluidic semi-automated system and lateral-flow assay). The assay was able to detect 20 different YFV strains with no cross-reactions with closely related viruses. […]