Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification
Author: Zachary Austin Crannell, Brittany Rohrman, and Rebecca Richards-KortumThis paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.
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Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription- RPA
Author: Tefera A. Mekuriaa, Shulu Zhangb, Kenneth C. EastwellaRT-RPA and lateral flow used to detect the Little cherry virus 2 coat protein gene. RT-RPA was simple, fast, and specific. The test has the sensitivity of RT-PCR and is more cost effective, being capable of detecting the virus from […]
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Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA
Author: Murinda SE, Ibekwe AM, Zulkaffly S, Cruz A, Park S, Razak N, Paudzai FM, Ab Samad L, Baquir K,Muthaiyah K, Santiago B, Rusli A, Balkcom SRPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.
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One-step digital plasma separation for molecular diagnostics
Author: Erh-Chia Yeh and Luke P. LeeFirst demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.
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Integrated Quantum Dot Barcode Smartphone Optical Device for Wireless Multiplexed Diagnostics
Author: Kevin Ming, Jisung Kim, Mia J. Biondi, Abdullah Syed, Kun Chen, Albert Lam, Mario Ostrowski, Anu Rebbapragada, Jordan J. Feld, and Warren C. W. ChanRPA is combined with quantum dot barcodes to allow multiplex detection of pathogens with a smartphone. RPA boosted the sensitivity of pathogen detection in a situation where PCR was not possible.
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Solid-phase RPA on microfluidic DVDs
Author: Luis A. Tortajada-Genaro, Sara Santiago-Felipe, Mary Amasia, Aman Russomb and Ángel MaquieiraaThe solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation.
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Centrifugal step emulsification for absolute quantification of nucleic acids by digital droplet RPA
Author: Friedrich Schuler, Frank Schwemmer, Martin Trotter, Simon Wadle, Roland Zengerle, Felix von Stetten and Nils PaustabThe centrifugal microfluidic droplet generation was used to perform the first digital droplet recombinase polymerase amplification (ddRPA). It was used for absolute quantification of Listerias monocytogenes DNA concentration standards with a total analysis time below 30 min – about a […]
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Real-time & label-free ring-resonator monitoring of solid-phase recombinase polymerase amplification
Author: Jonathan Sabaté del Ríoa, Tim Steylaertsb, Olivier Y.F. Henrya, Peter Bienstmanc, Tim Stakenborg“Development of a robust and automated solid-phase amplification platform for optical detection of dsDNA targets. Real-time and label-free monitoring of isothermal solid-phase amplification was achieved using ring resonators in combination with recombinase polymerase amplification. The amplification of a 144-bp target […]
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Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection
Author: Ahmed Abd El Wahed, Pranav Patel, Oumar Faye, Sasikanya Thaloengsok, Doris Heidenreich, Ponpan Matangkasombut, Khajohnpong Manopwisedjaroen, Anavaj Sakuntabhai, Amadou A. Sall, Frank T. Hufert, Manfred WeidmannReal-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4.
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Rapid and specific detection of Yam mosaic virus by reverse-transcription RPA
Author: Gonçalo Silvaa, Moritz Bömera, Chukwuemeka Nkereb, P. Lava Kumarb, Susan E. SealaYam mosaic virus (YMV; genus Potyvirus) is considered to cause the most economically important viral disease of yams (Dioscorea spp.) in West Africa which is the dominant region for yam production globally… The RT-exoRPA assay has, however, several advantages over the RT-PCR; positive […]