Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification

    Author: Zachary Austin Crannell, Brittany Rohrman, and Rebecca Richards-Kortum

    This paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.

  • Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription- RPA

    Author: Tefera A. Mekuriaa, Shulu Zhangb, Kenneth C. Eastwella

    RT-RPA and lateral flow used to detect the Little cherry virus 2 coat protein gene. RT-RPA was simple, fast, and specific. The test has the sensitivity of RT-PCR and is more cost effective, being capable of detecting the virus from […]

  • Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA

    Author: Murinda SE, Ibekwe AM, Zulkaffly S, Cruz A, Park S, Razak N, Paudzai FM, Ab Samad L, Baquir K,Muthaiyah K, Santiago B, Rusli A, Balkcom S

    RPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.

  • One-step digital plasma separation for molecular diagnostics

    Author: Erh-Chia Yeh and Luke P. Lee

    First demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.

  • Integrated Quantum Dot Barcode Smartphone Optical Device for Wireless Multiplexed Diagnostics

    Author: Kevin Ming, Jisung Kim, Mia J. Biondi, Abdullah Syed, Kun Chen, Albert Lam, Mario Ostrowski, Anu Rebbapragada, Jordan J. Feld, and Warren C. W. Chan

    RPA is combined with quantum dot barcodes to allow multiplex detection of pathogens with a smartphone. RPA boosted the sensitivity of pathogen detection in a situation where PCR was not possible.

  • Solid-phase RPA on microfluidic DVDs

    Author: Luis A. Tortajada-Genaro, Sara Santiago-Felipe, Mary Amasia, Aman Russomb and  Ángel Maquieiraa  

    The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation.

  • Centrifugal step emulsification for absolute quantification of nucleic acids by digital droplet RPA

    Author: Friedrich Schuler, Frank Schwemmer, Martin Trotter, Simon Wadle, Roland Zengerle, Felix von Stetten and Nils Paustab

    The centrifugal microfluidic droplet generation was used to perform the first digital droplet recombinase polymerase amplification (ddRPA). It was used for absolute quantification of Listerias monocytogenes DNA concentration standards with a total analysis time below 30 min – about a […]

  • Real-time & label-free ring-resonator monitoring of solid-phase recombinase polymerase amplification

    Author: Jonathan Sabaté del Ríoa, Tim Steylaertsb, Olivier Y.F. Henrya, Peter Bienstmanc, Tim Stakenborg

    “Development of a robust and automated solid-phase amplification platform for optical detection of dsDNA targets. Real-time and label-free monitoring of isothermal solid-phase amplification was achieved using ring resonators in combination with recombinase polymerase amplification. The amplification of a 144-bp target […]

  • Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection

    Author: Ahmed Abd El Wahed, Pranav Patel, Oumar Faye, Sasikanya Thaloengsok, Doris Heidenreich, Ponpan Matangkasombut, Khajohnpong Manopwisedjaroen, Anavaj Sakuntabhai, Amadou A. Sall, Frank T. Hufert, Manfred Weidmann

    Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4.

  • Rapid and specific detection of Yam mosaic virus by reverse-transcription RPA

    Author: Gonçalo Silvaa, Moritz Bömera, Chukwuemeka Nkereb, P. Lava Kumarb, Susan E. Seala

    Yam mosaic virus (YMV; genus Potyvirus) is considered to cause the most economically important viral disease of yams (Dioscorea spp.) in West Africa which is the dominant region for yam production globally… The RT-exoRPA assay has, however, several advantages over the RT-PCR; positive […]

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