Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Single-Step Recombinase Polymerase Amplification Assay Based on a Paper Chip for Simultaneous Detection of Multiple Foodborne Pathogens.
Author: Ahn H, Batule BS, Seok Y, Kim MG.A paper chip device-based recombinase polymerase amplification (RPA) method was developed for highly sensitive and selective single-step detection of foodborne pathogens. The diagnostic utility of the device was demonstrated by the reliable detection of E. coli and S.aureus present in […]
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Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil.
Author: Burkhardt A, Ramon ML, Smith B, Koike ST, Martin FN.RPA has been validated on over 200 infected strawberry plants with a diagnostic sensitivity of 93% and a diagnostic specificity of 99%, respectively. Together, this work demonstrates the value of using new approaches to identify loci for detection and provides […]
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Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses.
Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples.
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PrimedRPA: Primer design for Recombinase polymerase amplification assays.
Author: Higgins M, Ravenhall M, Ward D, Phelan J, Ibrahim A, Forrest MS, Clark TG, Campino S.PrimedRPA is a python-based package that automates the creation and filtering of RPA primers and probe sets. It aligns several sequences to identify conserved targets, and filters regions that cross react with possible background organisms.
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A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses
Author: Tian AL, Elsheikha HM, Zhou DH, Wu YD, Chen MX, Wang M, Chen D, Zhang XC, Zhu XQ.A recombinase polymerase amplification (RPA) assay combined with lateral flow (LF) strips was developed for the detection of N. caninum. The LF-RPA assay distinguished N. caninum from nine closely related protozoan species. The amplification reaction was completed in about 10 min […]
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Semi-quantitative nucleic acid test with simultaneous isotachophoretic extraction and amplification.
Author: Bender AT, Borysiak MD, Levenson AM, Lillis L, Boyle DS, Posner JD.We demonstrate simultaneous ITP and RPA can consistently detect 5 copies per reaction in buffer and 10 000 copies per milliliter of human serum with no intermediate user steps. We also show preliminary extraction and amplification of DNA from whole blood […]
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A novel method to detect meat adulteration by recombinase polymerase amplification and SYBR green I
Author: Cao Y, Zheng K, Jiang J, Wu J, Shi F, Song X, Jiang Y.At the isothermal temperature of 37 °C, RPA specifically identifies duck, chicken, cow, sheep and pig within 30 min of water bath. The RPA amplicons were successfully visualized by adding SG I. Furthermore, RPA can differentiate species of boiled, microwaved, high pressured […]
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Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick.
Author: Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Ys, Lu Lq.Herein, a rapid and convenient detection assay based on RPA with a lateral flow dipstick (LFD) was developed for detecting CyHV‐2. The highly conserved ORF72 of CyHV‐2 was targeted by specific and sensitive primers and probes. The optimized assay takes […]
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Rapid Authentication of Ginkgo biloba Herbal Products Using the Recombinase Polymerase Amplification Assay
Author: Liu Y, Wang XY, Wei XM, Gao ZT, Han JP.In this study, we developed a rapid identification method using the recombinase polymerase amplification (RPA) assay to detect two species, Ginkgo biloba and Sophora japonica(as adulteration), in Ginkgo biloba HPs.
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Efficacy of Recombinase Polymerase Amplification to Diagnose Trypanosoma cruzi Infection in Dogs with Cardiac Alterations from an Endemic Area of Mexico
Author: Jimenez-Coello M, Shelite T, Castellanos-Gonzalez A, Saldarriaga O, Rivero R, Ortega-Pacheco A, Garg N, Acevedo-Arcique C, Amaya-Guardia K, Melby P, Travi BL.The analytical sensitivity indicated that RPA-LF amplified T. cruzi DNA in samples containing almost equal to one to two parasites per reaction. Serial twofold dilutions of T. cruzi epimastigotes showed that the test had 95% (19/20) repeatability at concentrations of […]